This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually. Non-destructive annotations Overlays are the best way of annotating images in ImageJ (examples). Script A is publicly available at and Script B is available at. This synthesis of multimodal imaging may increase their potential diagnostic value. The image overlay: for ROIs that you only want to display. Optos UWF images can be successfully superimposed onto en-face OCTA images using freely available software that has been applied to ocular use. The main command for measuring in ImageJ is found under Analyze Measure (or just press M ). ![]() The scripts more easily allowed for automatic overlay of the images. The resulting warped Optos image was then successfully superimposed onto the ten Optos UWF images. The Optos UWF image could easily be transformed to the en-face OCTA images using BigWarp with common vessel branch point landmarks in the vasculature. The first algorithm was modified to include two scripts that automatically aligned all the en-face OCTA images. The images were then overlayed and stacked to create a series of ten combined Optos UWF and en-face OCTA images of increasing retinal depths. The Fiji plugin BigWarp was used to transform the Optos UWF image onto the en-face OCTA image using matching reference points in the retinal vasculature surrounding the macula. One image is a grayscale image from the brightfield acquisition. Basic rules for displaying fluorescence images: If you are only displaying one. Hi, I would like to overlay two images in ImageJ and failed up to now. En-face OCTA images were generated and ten (10) images at varying retinal depths were exported. 7.2 OVERLAY MULTIPLE FLUORESCENCE IMAGES. Optos UWF images and Heidelberg en-face OCTA images were collected from various patients as part of their routine care. ![]() ![]() To develop an algorithm and scripts to combine disparate multimodal imaging modalities and show its use by overlaying en-face optical coherence tomography angiography (OCTA) images and Optos ultra-widefield (UWF) retinal images using the Fiji (ImageJ) plugin BigWarp.
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